Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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light-activated signal transduction

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synthetic optogenetic transcription device

Multi-Component Switch

The synthetic optogenetic transcription device is a multi-component light-responsive signaling cascade that links melanopsin signal transduction to the nuclear factor of activated T cells (NFAT) control circuit to drive light-inducible transgene expression. It was reported to enable remote regulation of implanted transgenic cells in mice and to enhance blood-glucose homeostasis.

CFBacMamMusHumTxRep
Ev 37Rep 20Pr 49
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