Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
hGAVPO is a photo-activatable Gal4-based transcription factor used for light-controlled gene expression in mammalian systems. In the supplied evidence, it is identified only indirectly as a commonly used PA-Gal4 benchmark for comparison with the improved construct eGAV.
Synthetic cells were used as an encapsulation and delivery harness for orthogonal blue- and UV-light-activated DNA constructs in a cell-free expression context. In this reported use, they supported patterned, dual-wavelength control of RNA and protein expression and implementation of a light-controlled AND gate.