Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-controlled gene expression

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blue-light controlled gene modules

Construct Pattern
Since 2024light-controlled gene expression

Blue-light controlled gene modules are optogenetic expression constructs used with a portable smart blue-light controlled device to regulate local cytokine expression in the tumor microenvironment. In the cited 2024 MED study, this strategy was associated with enhanced activation of tumor-infiltrating immune cells and remodeling of an immunosuppressive tumor milieu.

CFBacMamMusHumTxRep
Ev 58Rep 9Pr 59

affinity-based purification of caged DNA

Engineering Method
Since 2013affinity-based separationchemical caging of dnalight-controlled gene expression

Affinity-based purification of caged DNA is a preparation method for caged linear double-stranded DNA in which a minimal protein-expression cassette is reacted with Bio-Bhc-diazo and the caged DNA is isolated by affinity separation. The reported application context is light-controlled gene expression in mammalian cells.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71

lentiviral platform

Delivery Strategy
Since 2021light-controlled gene expression

The lentiviral platform is a delivery harness used to implement an optimized Light-On system for light-controlled gene expression in neurons. The available evidence supports its use for optically regulated transgene expression in neuronal contexts, but does not provide further construct-level or performance details.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

red/far-red light-responsive gene switch

Multi-Component Switch
Since 2014light-controlled gene expressionorthogonal multi-channel regulation

The red/far-red light-responsive gene switch is a mammalian optogenetic expression system used as one channel in a triple-light gene control framework. The supplied evidence supports that it can function orthogonally with UV-B- and blue-light-responsive gene switches in a single mammalian cell culture.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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