Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
4 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-4 of 4
Set2-LANS is an optogenetic fusion of the yeast H3K36 methyltransferase Set2 with the light-activated nuclear shuttle (LANS) domain. It provides rapid and reversible light control of Set2, enabling inducible nuclear localization and control of H3K36 methylation in vivo.
The synthetic transcription factor is an engineered cargo used with a red/far-red light-regulated phytochrome-based nuclear localization system. In mammalian cells and zebrafish, its light-controlled nuclear translocation was used to drive transgene expression as a functional readout of regulated protein import.
Light-controlled nuclear localization is an optogenetic engineering method reported for constructing repressors of gene expression in yeasts. It uses light to regulate nuclear localization, thereby controlling transcriptional repression.
Light-inducible nuclear localization signals are engineered protein-domain tools that enable light-dependent control of nuclear import in living cells. The available evidence supports their use for precise spatiotemporal regulation of protein dynamics through control of subcellular localization.