Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

2 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

Sort
light-controlled regulation of gene expression

Showing 1-2 of 2

Loaditems
Page 1 / 1

blue light-responsive and rapidly reversible expression system

Multi-Component Switch

The blue light-responsive and rapidly reversible expression system is an optogenetic multi-component gene switch for mammalian cells that enables blue light-controlled transgene expression. It was developed as a rapidly reversible blue-light module and used within an orthogonal UV-B/blue/red-far-red triple-gene control framework.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37

light-controlled biocatalytic one-pot system

Multi-Component Switch

The light-controlled biocatalytic one-pot system is a multi-component biocatalytic platform developed for precise regulation of enzymatic decarboxylation. In the reported system, illumination controls gene expression and photocatalysis within a one-pot format, and the system achieves high conversion and stereoselectivity.

CFBacMamMusHumTxRep
Page 1 / 1