Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
Cytoplasmically diffuse CRY2 with membrane-anchored CIBN is a proof-of-concept CRY2/CIB optogenetic configuration adapted into Drosophila-oriented vector systems. It was proposed to use blue light to recruit CRY2 fusion proteins, including a possible CRY2-Rho1 fusion, to the plasma membrane.
The CRY2/CIB system is a blue-light-responsive optogenetic multi-component switch used for gene regulation and control of protein recruitment. The supplied evidence identifies it as a well-known optogenetic system and indicates that blue light can recruit CRY2-fused cargo to membrane-anchored CIB for spatial and temporal control of downstream cellular events.
The Rho1-CRY2 fusion construct is a proposed optogenetic multi-component switch for Drosophila in which a small G protein Rho variant would be fused to CRY2. The intended function is blue-light-dependent recruitment of Rho1-CRY2 to membrane-anchored CIB to control subcellular localization and downstream events, but the available evidence indicates the construct was still being cloned rather than functionally validated.