Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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light-controlled subcellular targeting

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organelle-targeting strategies

Engineering Method

Organelle-targeting strategies are optogenetic engineering approaches that use light to direct proteins to specific subcellular compartments and regulate subcellular organization. Reported applications include manipulation of nuclear translocation, plasma membrane morphology, and control over assembly of synthetic organelles.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59
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