Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
FUN-LOVSP is a single-plasmid blue-light-responsive variant of the FUN-LOV optogenetic switch developed in yeast. It was generated by reconfiguring the original system with promoter and terminator replacements and supports light-induced reporter expression in selectable-marker derivatives including Nat- and Hph-marked formats.
The red/far-red light-responsive bi-stable toggle switch is a multicomponent optogenetic gene control system for mammalian cells. It uses red and far-red light to regulate gene expression with temporal and spatial control, and was reported as the first such red/far-red light-triggered gene switch for mammalian cells.
PA-Cre is an optogenetic Cre recombinase system for light-controlled site-specific recombination. In the supplied evidence, it is one of three bacterial optogenetic Cre variants systematically compared for population-level and single-cell activity.