Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

3 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-dependent activation

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light-controlled crRNA

RNA Element
Since 2020light-dependent activationPhotocleavagesteric inhibition of target dna associationFunctional AssaySequence Verification

Light-controlled crRNA is a chemically modified CRISPR guide RNA in which vitamin E is attached to the 5' terminus through a photolabile linker, creating a caged crRNA that inactivates CRISPR/Cas9 until light exposure. Upon irradiation, this design restores CRISPR/Cas9 activity and supports genome editing of VEGFA and knockdown of EGFP expression in human cells.

CFBacMamMusHumTxRep
Ev 55Rep 9Pr 59

optoWnt optogenetic system

Multi-Component Switch
Since 2019activation of canonical wnt/beta-catenin signalingcanonical wnt/beta-catenin pathway activationlight-dependent activationoptogenetic light-dependent activation

The optoWnt optogenetic system is a light-controlled multi-component switch that activates canonical Wnt/beta-catenin signaling. In human embryonic stem cells, it produced a reported dynamic range greater than 500-fold and drove transcriptional and mesendoderm differentiation responses.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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