Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
The light-activatable FGF receptor is a genetically encoded construct expressed in an epithelial cell line to control MAPK/ERK signaling with light. In the cited study, it functioned as the optical input module for stimulating the pathway during information transmission measurements under random light pulse trains.
OptoR2 is a light-activated FGFR2 variant engineered to selectively activate signaling by the major fibroblast growth factor receptor in keratinocytes. Upon illumination, it induces FGFR signaling and, in OptoR2-expressing HEK293T cells, promotes migration and proliferation with temporal precision.
Opto-mGluR6 is a chimeric optogenetic receptor designed for retinal ON-bipolar cells. It combines light-sensing domains from melanopsin with intracellular domains from mGluR6 to couple light activation to mGluR6-like GPCR signaling.