Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

8 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-dependent regulation

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light-engineering technology

Engineering Method
Since 2022Degradationlight-dependent regulationlight-dependent regulation of plant defense signalingprotein degradation

Light-engineering technology, as described in the cited review, is an engineering approach that uses environmental light manipulation with light-emitting diodes (LEDs) to modulate plant defense. The review presents it as a potential strategy, alongside genome engineering, for fine-tuning crop defense and yield.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

optogenetic regulation

Engineering Method
Since 2018light-dependent regulationspatiotemporal control of signaling

Optogenetic regulation is a light-input engineering method used to manipulate cellular signaling with spatiotemporal precision. In the cited review, it is specifically described as providing insights into the spatiotemporal control of RAS/MAPK and phosphoinositide-3 kinase (PI3K) pathways.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

photoreceptors

Protein Domain
Since 2022light-dependent regulationlight sensingComputational Design

Photoreceptors are naturally occurring or engineered photosensitive protein domains that respond to light at varying wavelengths and function as light-regulated actuators in optogenetics. They have been used to confer genetically encoded, light-dependent control of cellular functions, including regulation of gene expression in bacteria.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

use of exogenous chromophores

Engineering Method
Since 2021light-dependent regulation

Use of exogenous chromophores is an implementation consideration in mammalian optogenetic gene regulation systems relevant to clinical applications. The cited review identifies externally supplied chromophores, and their effects on cell biology, as an unresolved factor affecting light-controlled gene and protein expression.

CFBacMamMusHumTxRep
Ev 20Rep 9Pr 59

Opto-Kv1

Multi-Component Switch
Since 2021dominant-negative regulationlight-dependent regulationlov-domain photoswitching

Opto-Kv1 is a photoactivatable voltage-gated potassium channel generated by fusing the light-sensitive LOV domain from Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit. It enables acute light-dependent regulation of Kv1 channel expression/function, and the Opto-Kv1(V400D) variant can down-regulate Kv1 currents in a blue light-dependent manner.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37

YF1-FixJ-PhlF

Multi-Component Switch
Since 2025blue light activationlight-dependent regulation

YF1-FixJ-PhlF is a blue light-activated induction module incorporated into a dual light-controlled co-culture system. In the cited system, it serves as one light-responsive control layer for regulating population composition.

CFBacMamMusHumTxRep
Ev 28Rep 0Pr 37
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