Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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light-dependent regulation via an aslov2 photoreceptor

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photosensitive degron psd3

Protein Domain

psd3 is a variant of the photosensitive degron built around an AsLOV2 photoreceptor module for light-dependent control of protein abundance. Available evidence indicates that its light sensitivity can be tuned by photocycle mutations and that it was developed in a system for optogenetic regulation of transcription-related processes.

CFBacMamMusHumTxRep
Ev 16Rep 9Pr 59
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