Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
6 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-6 of 6
This tool is a genetically encoded optogenetic switch that uses light to regulate biological function. The supplied evidence supports its use to control central metabolic flux in Escherichia coli and to drive spatiotemporally controlled shape change in yeast with pulses of dim blue light.
LOV-TRAP is a light-controlled multi-component optogenetic tool used in the cited study for upstream activation of Rac1. In that application, it enabled optical perturbation of Rac1 signaling concurrently with near-infrared FRET imaging of Rho GTPase activity.
opto-PKR is an engineered photo-switchable version of the integrated stress response sensor kinase PKR that enables light-based virtual control of ISR signaling. In the cited 2022 study, controlled light activation of opto-PKR was used to drive ISR activity and reveal input-proportional transcriptional dynamics.
psRhoGEF is a photoswitchable RhoA guanine exchange factor designed to precisely control endogenous RhoA activity with light. It was used to impose rheostatic optical control of RhoA signaling amplitude and thereby elicit distinct cellular responses.
The LOV system is a well-known optogenetic light-responsive system used for gene regulation. The supplied evidence places it within optogenetic control of gene expression in mammalian-cell contexts relevant to clinical applications.
Optogenetic tools are genetically encoded, light-responsive systems that enable control of biological processes with the spatiotemporal precision of light. In the cited context, they are described specifically as light-controlled gene expression technologies in mammalian cells.