Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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The YF1/FixJ system is a blue light-repressible multi-component optogenetic switch evaluated for transcriptional control in the cyanobacterium Synechococcus sp. PCC 7002. In the cited PCC 7002 study, it was tested as a heterologously expressed light-input gene regulation system and showed limited performance, with a reported maximum dynamic range of 1.5-fold.
The light-repressive promoter PGAP-pLRE was strictly suppressed by light, with expression capacity comparable with PGAP in darkness.
The anchor review explicitly names PULSE as a plant-usable light-switch system combining EL222-based repression with a red-light-inducible activator architecture.