Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Photoactivatable CRISPR-Cas9 is an optogenetic genome-editing construct pattern in which Cas9-mediated gene perturbation is controlled by light. It has been applied in mice to induce LIF knockdown under light-emitting diode illumination, enabling spatiotemporal perturbation of embryo implantation-associated biology and fertility.
Human Neuropsin (hOPN5) is a light-responsive optogenetic switch used to selectively control Gq signaling. It has been reported to support specific and repetitive manipulation of this pathway in vitro and in vivo with high spatiotemporal resolution.