Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
We show that expression and activation of either ChR2D156A, a blue-light activated cation channel ... both of which hyperpolarize cells, significantly lowers the incidence of KRAS tumor formation.
Furthermore, we provide an overview of junction- and cytoskeletal-related biosensors and optogenetic probes that have contributed to these advances...
Microbial opsins are genetically encoded seven-transmembrane proteins from diverse microorganisms that render cells light responsive by transporting ions across cellular lipid membranes. In optogenetics, they are used as molecular sensitizers to activate or silence neural activity with brief pulses of light.
Light-gated ion channels and pumps are optogenetic actuators that use light to control transmembrane ion flux. They have been applied to probe dynamic ion signalling cascades in animals and plants and to increase ATP accumulation for improved biomass or bioproduct yields in microorganisms.
We also review spectrally shifted variants offering possibilities for enhanced depth of tissue penetration, combinatorial stimulation for targeting different cell subpopulations, or all-optical read-in and read-out studies.