Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

2 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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light-gated protein-protein interaction

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Cry2/CIB is a genetically encoded blue-light-activated protein dimerization module derived from Arabidopsis thaliana. It is used to optically induce protein-protein interactions and has been applied to control transcription, protein localization, protein secretion, and, when coupled to BAX, light-triggered apoptosis.

CFBacMamMusHumTxRep
Ev 37Rep 20Pr 49

light-gated protein-protein interaction system

Multi-Component Switch

This tool is a light-gated protein-protein interaction system engineered to regulate the activation pattern of the Raf/MEK/ERK signaling pathway. It enables light-mediated kinetic control of intracellular signaling and was used in rat PC12 pheochromocytoma cells to induce neurite outgrowth without growth factors.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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