Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-gated restoration of transcription factor binding

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caged NF-κB DNA decoy

RNA Element
Since 2011light-gated restoration of dna:dna hybridizationlight-gated restoration of transcription factor bindingPhotocleavagetranscriptional decoy sequestration of nf-κb

The caged NF-κB DNA decoy is a photoregulated oligonucleotide decoy in which photoremovable protecting groups are installed on nucleobases within an NF-κB decoy sequence. UV irradiation removes the caging groups, restores decoy hybridization and NF-κB binding activity, and enables light-controlled regulation of NF-κB-driven transcription in mammalian cells.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59
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