Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-induced homodimerization

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TAEL

Protein Domain
Since 2020dna binding to the c120 promoter elementHeterodimerizationlight-induced dimerizationlight-induced homodimerizationtranscriptional activation

TAEL is an engineered optogenetic transcription factor optimized for zebrafish in which blue light induces TAEL dimerization, binding to the C120 promoter element, and activation of downstream transcription. TAEL 2.0 is an improved transgenic implementation that enables inducible expression at late embryonic and larval stages and produces faster, higher reporter expression than the original system.

CFBacMamMusHumTxRep
Ev 25Rep 20Pr 71

aureochrome 1 LOV-domain-based optical TrkB activation approach

Protein Domain
Since 2019Heterodimerizationlight-induced homodimerization

The aureochrome 1 LOV-domain-based optical TrkB activation approach is an optogenetic TrkB activation strategy built around the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida. It was presented as a demonstration that optical TrkB activation can be implemented with an optical homo-dimerizer other than CRY2.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

pdDronpa1.2

Protein Domain
Since 2025Heterodimerizationlight-induced homodimerization

pdDronpa1.2 is a protein domain used in a single-construct optogenetic talin system to enable light-inducible C-terminal homodimerization. In the cited 2025 study, this light-triggered talin dimerization was sufficient to drive talin recruitment to adhesion sites, adhesion formation, coupling to actin retrograde flow, and downstream mechanosignaling.

CFBacMamMusHumTxRep
Ev 28Rep 0Pr 59

single-construct optogenetic talin

Multi-Component Switch
Since 2025Heterodimerizationlight-induced homodimerizationmechanosignaling activationoptogenetic control of protein localization

Single-construct optogenetic talin is an engineered light-responsive talin system in which pdDronpa1.2 enables light-inducible C-terminal talin homodimerization. In the cited study, this induced talin recruitment to adhesion sites, promoted adhesion formation, engaged actin retrograde flow, and activated downstream mechanosignaling.

CFBacMamMusHumTxRep
Ev 28Rep 0Pr 37
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