Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
The optogenetic Amyloid-β2 peptide is a fluorescently tagged construct, also referred to as Aβ2-CRY2-mCherry, designed for blue-light-controlled oligomerization of an amyloid-β species in vivo. It enables inducible amyloid-β oligomerization for neurodegeneration-related studies.
The CRY2-mCherry-Drosophila β-catenin optogenetic switch is a fusion protein comprising Arabidopsis thaliana CRY2, mCherry, and Drosophila β-catenin. Blue light induces oligomerization of the fusion protein, which inhibits downstream Wnt signaling in vitro and in vivo and enables temporal inactivation of β-catenin.