Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-induced photobody formation

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AtCRY2-TopBP1 optogenetic DNA damage checkpoint switch

Multi-Component Switch
Since 2020light-induced photobody formationlight-induced protein degradationoptogenetic activation of dna damage signalingprotein clustering/assembly

The AtCRY2-TopBP1 optogenetic DNA damage checkpoint switch is a fusion of Arabidopsis thaliana cryptochrome 2 (AtCRY2) to the DNA damage checkpoint protein TopBP1. In mammalian nuclei, light-induced AtCRY2 photobody formation is used to activate DNA damage signaling in the absence of DNA damage.

CFBacMamMusHumTxRep
Ev 36Rep 9Pr 37
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