Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
BcLOVclust is a cytoplasmic BcLOV4-derived protein domain engineered for light-controlled intracellular clustering. It enables optogenetic clustering in mammalian cells and has been applied to control signaling proteins and stress granules.
Clustering Indirectly using Cryptochrome 2 (CLICR) is a genetically encoded optogenetic method for spatiotemporal control of endogenous transmembrane receptor activation. It uses engineered Arabidopsis Cry2 to regulate target receptor clustering through noncovalent interactions, thereby activating downstream signaling.
The light-induced co-clustering assay is an optogenetic functional assay used to assess protein-protein interactions in Drosophila S2 cells. It was reported in the context of light-induced protein clustering and uses co-clustering as the interaction readout.
OptoCNK1 is an optogenetic CNK1 clustering construct implemented in MCF7 cells to stimulate CNK1 independently of upstream effectors. Light-induced CNK1 clustering was reported to selectively engage RAF-MEK-ERK or AKT signaling as a function of applied light intensity, with corresponding effects on cell fate.
Explicitly supported tool/component names found in these sources include Cph1, CRY2olig, EL222, LOV2, OptoAMP, and PULSE.