Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
The photosensitive degron (psd) is a protein-domain tool that confers acute light-induced degradation on fused proteins. Available evidence indicates activity in the nervous system and a construct requirement that the psd be placed at the carboxy terminus of the target protein.
The blue light-inducible degradation (B-LID) domain is a light-activated degron used to trigger loss of a fused protein in vivo. Available evidence indicates that it must be fused to the carboxy terminus of the target protein and can elicit light-dependent loss of Cactus function in developing Drosophila embryos.
The AtCRY2-TopBP1 optogenetic DNA damage checkpoint switch is a fusion of Arabidopsis thaliana cryptochrome 2 (AtCRY2) to the DNA damage checkpoint protein TopBP1. In mammalian nuclei, light-induced AtCRY2 photobody formation is used to activate DNA damage signaling in the absence of DNA damage.