Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-induced protein heterodimerization

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CRY2/CIB1 system

Multi-Component Switch
Since 2017kinetic modulation of cry2/cibn binding by green lightlight-controlled subcellular relocalizationlight-induced protein heterodimerization

CRY2/CIB1 is a light-responsive multi-component switch derived from Arabidopsis thaliana that uses CRY2-CIB1 association to control protein localization and kinase regulation. It has been reported to rapidly redirect proteins within living E. coli cells and to serve as the basis for light-regulated protein kinase designs.

CFBacMamMusHumTxRep
Ev 37Rep 20Pr 49

CRY2/CIB system

Multi-Component Switch
Since 2021light-controlled subcellular recruitmentlight-induced protein heterodimerization

The CRY2/CIB system is a blue-light-responsive optogenetic multi-component switch used for gene regulation and control of protein recruitment. The supplied evidence identifies it as a well-known optogenetic system and indicates that blue light can recruit CRY2-fused cargo to membrane-anchored CIB for spatial and temporal control of downstream cellular events.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37

DNMT3A-CRY2/CIB1-TRF1 optogenetic telomere-targeting fusion construct system

Multi-Component Switch
Since 2016dna methylationlight-induced protein heterodimerizationtargeted subcellular localizationComputational Design

The DNMT3A-CRY2/CIB1-TRF1 system is a blue-light-responsive multi-component optogenetic construct that recruits human DNMT3A to telomeric regions through CRY2-CIB1 interaction and TRF1-mediated telomere binding. In the reported HeLa cell configuration, light activation selectively increased subtelomeric CpG methylation at six examined chromosome ends and was associated with progressive telomere lengthening over three cell generations.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 37
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