Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
FUN-LOV is a fungal light-oxygen-voltage optogenetic switch for yeast built from Neurospora crassa photoreceptors WC-1 and VVD. It uses the photon-regulated interaction of these components to drive light-dependent target gene activation, including GPD1 and ADH1 expression in a wine yeast strain.
GFP-PHR-caspase8/Flag-CIB1N-caspase8 is a blue light-responsive two-component optogenetic caspase-8 system composed of GFP-PHR-caspase8 and Flag-CIB1N-caspase8 fusion proteins. Under blue light, the two components interact more strongly and undergo light-dependent cleavage, consistent with inducible caspase-8 activation.
Light-inducible transcriptional effectors (LITEs) are an optogenetic two-hybrid system that combines a customizable TALE DNA-binding domain with Arabidopsis thaliana CRY2 and its interacting partner CIB1. The system enables light-triggered, reversible control of endogenous mammalian gene expression and targeted epigenetic chromatin modification.