Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
opto-iTrkA is an iLID-based light-activated TrkA construct engineered to control TrkA receptor signaling with blue light. The intracellular TrkA module is cytosolic, monomeric, and inactive in the dark, and activation requires light-driven recruitment in the presence of tdnano to reproduce downstream ERK and Akt signaling.
opto-iTrkB, also called iLID opto-iTrkB, is a blue-light-activated TrkB receptor tyrosine kinase construct built on the improved Light-Induced Dimerizer system. In the reported design, cytosolic inactive iLID-RTK is recruited to tdnano under blue light, which drives receptor dimerization and activates downstream ERK and Akt signaling.
tdnano is a constructed tandem-dimer of the iLID binding partner nano used as the second component of a blue-light-responsive iLID system. In the reported opto-receptor tyrosine kinase designs, blue light drives recruitment of two iLID-fused RTK molecules to tdnano, enabling receptor dimerization and activation.