Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
5 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-5 of 5
This tool is a tunable optogenetic, light-inducible gene expression system used to control morphogen production in vitro. It was reported to generate long-range Sonic hedgehog (Shh) gradients that pattern neural progenitors into spatially distinct progenitor domains resembling vertebrate neural tube organization in vivo.
Lockdown is a blue light-operated, multi-component CRISPR/Cas13b system for optical control of RNA abundance in mammalian cells. It uses a blue light-dependent switch to induce CRISPR/Cas13b expression, enabling sequence-specific knockdown of exogenous and endogenous mRNA.
The blue light-responsive Cas13b mRNA knockdown system is an optogenetic RNA-control platform in which blue light induces expression of an engineered Cas13b that specifically degrades target mRNAs. In the reported application, combining three blue light-inducible switches reduced protein levels by more than 99%.
Here we show the effects of photo-inducible complementation in the albino Arabidopsis mutant pap7-1 using a red/blue optoswitch with monochromatic LEDs. Expression of a blue-light-induced PAP7 construct that is silent under red light reconstitutes PEP... The optoswitch termed blue-light valved biogenesis opens experimental avenues to study non-viable plant mutants.
The blue light-dependent switch is a multi-component optogenetic control element used in the Lockdown system to induce expression of CRISPR/Cas13b in response to blue light. In this context, it enables light-triggered control of RNA levels through Cas13b-mediated mRNA knockdown.