Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

2 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-inducible oligomerization

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light-inducible oligomerization of Eps15

Engineering Method
Since 2019assembly-state tuning between liquid-like and solid-like protein assemblieslight-inducible oligomerizationOligomerization

Light-inducible oligomerization of Eps15 is an optogenetic engineering method used to tune Eps15 initiator-protein assembly strength in real time during endocytosis. In mammalian Eps15 knockout cells, low light produced liquid-like Eps15 assemblies that restored normal endocytic rates, whereas higher light produced solid-like assemblies that stalled vesicle budding.

CFBacMamMusHumTxRep
Ev 36Rep 9Pr 59
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