Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
4 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-4 of 4
The optimized CRISPR-Cas9-based light-inducible gene expression device is a multi-component light-on transcription system built on CRISPR-Cas9-based engineering. It was reported to control gene transcription in a dose-dependent manner and was applied to induce P53 expression in bladder cancer cells.
Opto-CRISPR-p300 is a light-inducible CRISPR-based epigenome activation system. In the cited 2022 study, it induced Egr3 and Nab2 transcripts and produced bidirectional transcriptional regulation in D1-MSNs and D2-MSNs.
GAVPO is a light-inducible transactivator used as an optogenetic transcriptional control module. In zebrafish embryos, it was combined with genetically encoded cell-ablation technologies to drive targeted cell ablation with spatial and temporal control.
Here, we present a new optogenetic CRISPR tool (Blue Light-inducible Universal VPR-Improved Production of RGRs, BLU-VIPR)...