Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
Phase-separation-engineered optogenetic synthetic transcription factors are light-inducible transcription factor constructs modified with intrinsically disordered domains to promote liquid-liquid phase separation. In a 2021 study, this design increased transcriptional activation in mammalian cells and in mice after in situ transfection.
WC-1 is a LOV blue-light photoreceptor from the fungus Neurospora crassa that has been used with the partner photoreceptor VVD in the FUN-LOV optogenetic switch. In Saccharomyces cerevisiae, the WC-1/VVD pair mediates photon-regulated interaction to drive light-inducible transcriptional control and associated phenotypes such as flocculation.
iLiEX is a genome-integrative, single-copy light-inducible expression system developed in Streptomyces griseus NBRC 13350 that combines LitR-based light-responsive control, a T7 RNA polymerase transcriptional amplification module, and serine integrase-mediated genome integration. It enables light-controlled transcription from a chromosomally integrated construct in Streptomyces.