Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
BOS is a bifunctional optogenetic transcriptional control system developed in Escherichia coli by combining the light-inducible transcriptional regulator LEVI mutant with the chemical-inducible repressor LacI. It enables blue-light-responsive regulation of target genes and has been applied to metabolic engineering for shikimic acid and l-valine production.
As demonstrated with GFP, the light-inducible promoter 4pLRE-cPAOX1 was 70 % stronger than the constitutive promoter PGAP, with L/D ratio = 77.
The cyp-14A5 promoter is a light-responsive Caenorhabditis elegans promoter used as a construct element for visible light-inducible transcription. In the cited study, it drove ectopic gene expression in non-neuronal tissues and enabled induction of synthetic light-induced sleep and rapid aging phenotypes.