Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Opto-nanobodies (OptoNBs) are chimeric photoswitchable nanobody-based proteins whose binding to proteins of interest is enhanced or inhibited by blue light illumination. They function as a reversible, multi-component light-controlled binding switch for regulating protein interactions.
The monobody non-immunoglobulin scaffold was reported as a platform for engineering light-responsive protein binding. The available evidence supports that this scaffold can be configured for optically controlled binding, but does not specify the target, photoreceptor architecture, or quantitative performance.