Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

4 items matching 1 filter

Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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light-responsive transcriptional regulation

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demultiplexer for dynamically encoded signals

Construct Pattern
Since 2021crispri/dcas9-based transcriptional regulationdynamic signal decodingfalling-edge pulse detectionkinetic filteringlight-responsive transcriptional regulationtemporal demultiplexing

The demultiplexer for dynamically encoded signals is a synthetic gene circuit architecture that decodes temporally encoded inputs into distinct gene-expression outputs. In a 2021 study, this circuit was shown to demultiplex dynamic signals and was further combined with dCas9-based gene networks to build pulsatile-signal filters and decoders.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71

chalcone synthase promoter

RNA Element
Since 1998light-responsive transcriptional regulation

The chalcone synthase promoter is a plant light-responsive promoter used to control expression of a foreign gene in tobacco BY-2 cells. The available evidence supports its function as a promoter-based light-regulated gene expression tool in this heterologous plant cell context.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 59

dual light-controlled co-culture system

Multi-Component Switch
Since 2025light-responsive transcriptional regulationquorum sensing-mediated intercellular communicationtoxin-antitoxin-mediated population control

The dual light-controlled co-culture system is a multi-component optogenetic platform for regulating microbial population composition in co-culture. It combines an optimized CcaS/R module with a blue light-activated YF1-FixJ-PhlF module, together with orthogonal quorum sensing and a toxin-antitoxin effector layer.

CFBacMamMusHumTxRep
Ev 28Rep 0Pr 37
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