Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
8 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-8 of 8
pc-PROTAC1 is a photocaged PROTAC construct designed for light-dependent targeted protein degradation in live cells. In the cited study, it exhibited potent degradation activity only after light irradiation, establishing a light-activated degradation strategy.
Photo-caged PROTACs (pc-PROTACs) are light-activated proteolysis-targeting chimeras designed to trigger targeted protein degradation only after irradiation. The reported study presented pc-PROTACs as a general strategy for inducing degradation activity with light and showed that pc-PROTAC1 was potently active in live cells only after light exposure.
Photocontrollable nucleic acid displacement reaction is a light-gated nucleic acid engineering method used within a near-infrared-activatable cascade recycling amplification system. In the cited implementation, it is integrated with exonuclease III-assisted nucleic acid amplification and upconversion nanoparticles to enable spatiotemporally controllable, signal-amplified mRNA imaging in selected living cancer cells.
An upconversion nanoparticle is used as a light-transducing component in an immunodevice that converts near-infrared input into activation of a UV light-activatable immunostimulatory agent. In the reported 2019 Nature Communications system, this enabled remote and spatially selective control of antitumor immunity in vitro and in vivo.
Moreover, we highlight the principal guidelines for the development of light-responsive materials and photosensitive compounds.
The upconversion nanoparticle-based immunodevice is a multi-component, light-activatable immunodevice for remote optical control of antitumor immunity in vitro and in vivo. It combines a rationally designed UV light-activatable immunostimulatory agent with an upconversion nanoparticle that transduces near-infrared light sensitivity into device activation.
While light-actuated chemicals such as photolabile 'caged' compounds have been in existence for decades
Stimulus-responsive nanomedicines are another trend accompanying both targeting strategies to address further issues of tissue penetration, cellular internalization, and drug release that are critical for the payload's therapeutic efficacy