Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
7 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-7 of 7
Biofunctional nanodot arrays (bNDAs) are nanoscale surface-patterned delivery harnesses designed to spatially control dimerization and clustering of cell-surface receptors. In live cells, they were used to capture extracellularly GFP-tagged Lrp6 and drive assembly of active Wnt signalosomes at the plasma membrane.
Intrinsically disordered regions (IDRs) are protein domains used in engineered synthetic condensates to drive constitutive oligomerization and cluster formation. In the cited modular membraneless organelle design, IDR-mediated assembly is separated from cargo recruitment by fused interaction domains, enabling tunable control of condensate composition and function.
The tau microtubule-binding domain (MTBD) is a tau protein domain that has a distinct but complementary role with the tau polyproline-rich domain in cellular liquid-liquid phase separation. Evidence further indicates that when MTBD is absent, the tau polyproline-rich domain co-condenses with the microtubule plus-end tracking protein EB1.
The tau polyproline-rich domain (PRD) is a tau-derived protein domain that promotes liquid-liquid phase separation in cells. Available evidence indicates that its condensate-forming activity is regulated by phosphorylation and that, in the absence of the microtubule-binding domain, it can co-condense with EB1.
coacervate droplets produced by liquid-liquid phase separation have emerged as an alternative membrane-free compartmentalization paradigm
Phase-separation-engineered optogenetic synthetic transcription factors are light-inducible transcription factor constructs modified with intrinsically disordered domains to promote liquid-liquid phase separation. In a 2021 study, this design increased transcriptional activation in mammalian cells and in mice after in situ transfection.
Furthermore, we design hormone-responsive liquid-liquid phase-separated (LLPS) condensates that strongly amplify transcription when exceeding a critical interaction threshold. These functional LLPS condensates provide a tunable platform for transcriptional control with up to several hundred-fold activation.