Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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local activation of receptor signaling

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CRY2-CIB1 receptor optogenetic activation system

Multi-Component Switch
Since 2024light-induced heterodimerizationlocal activation of receptor signalingmembrane recruitment of receptor intracellular domains

The CRY2-CIB1 receptor optogenetic activation system is a light-responsive multi-component switch based on the light-induced interaction between Cryptochrome 2 (CRY2) and CIB1. It was implemented as a bicistronic vector co-expressing CRY2 fused to the intracellular domain of a guidance receptor together with membrane-anchored CIB1, enabling local optical activation of receptor signaling in neurons.

CFBacMamMusHumTxRep
Ev 37Rep 9Pr 37
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