Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

Concrete methods used to design, build, verify, or characterize engineered systems.

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motif sufficiency for signaling output

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GUS-NC80 fusion protein

Construct Pattern

The GUS-NC80 fusion protein is a transgenic plant construct in which β-glucuronidase is fused to the 80-residue NC80 motif from Arabidopsis CRY2. In the cited study, this fusion was constitutively active despite being unphosphorylated, supporting that NC80 is sufficient to confer CRY2 physiological function when derepressed.

CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
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