Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Deg-LITer is a multi-component optogenetic gene circuit in which the TetR repressor is fused to a degradation tag through the LOV2 light-sensitive domain. It is part of the LITer toolset for light-controlled regulation in mammalian cells.
Light-Inducible Tuner (LITer) is a mammalian optogenetic gene-circuit platform in which TetR is fused through the LOV2 light-sensitive domain to either a Tet-inhibitory peptide or a degradation tag. It enables light-controlled negative-feedback regulation of gene expression and was reported to reduce expression noise while providing tunable output control.