Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Recombinant AAV1/2 viral particles are a viral gene delivery harness used to express the LOV2-PAH1 optogenetic REST inhibitor in HEK293T cells, primary neurons, and mouse hippocampal neurons in vivo. In the cited study, these particles enabled efficient hippocampal neuronal transduction and subsequent functional testing of the delivered cargo.
The web research summary states that the anchor paper describes an adult human neocortical acute-and-cultured slice platform optimized for rapid molecular-genetic manipulation, emphasizing short-latency HSV-1 neuronal transduction, and lists HSV-1 amplicon vectors as the core genetic-delivery component.