systematic in situ hybridization
Assay MethodSystematic in situ hybridization mapped specific clusters to the principal DR, caudal DR, or MR.
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
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Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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Systematic in situ hybridization mapped specific clusters to the principal DR, caudal DR, or MR.
We used DNA array technology to decipher the interplay between these responses in P. putida KT2440 subjected to a short challenge (15 min) with toluene.
The Affymetrix ATH1 microarray is a transcript expression assay for genome-scale expression profiling in Arabidopsis thaliana. In the cited AtGenExpress study, it was used to generate a comprehensive transcript dataset across abiotic stress conditions including UV-B light, drought, and cold.
A cDNA microarray is a gene-expression profiling assay used in Arabidopsis to examine how phyA pathway mutations affect far-red light control of genome-wide expression. In the cited study, it was applied to profile transcriptional responses associated with phytochrome A signaling and to compare mutant expression patterns.
An expressed sequence tag-based microarray is a microarray expression-profiling assay used to measure genome-scale transcript patterns in Arabidopsis. In the cited study, it was applied to profile gene expression underlying light-controlled development across different light conditions.