Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-3 of 3
Light-emitting diode illumination is a light-delivery harness used with a photoactivatable CRISPR-Cas9 system to provide optical input for temporally and spatially controlled genome editing in mice. In the cited in vivo study, it was applied in the context of embryo implantation and reproductive function.
We developed transgenic mice expressing optical actuators ... photoactivated adenylyl cyclase (PAC) in smooth muscle actin (SMA)-negative mural cells that lack the machinery for SMA-mediated vasoregulation.
This tool is a generalised computational modeling technique for simulating optogenetic mechanisms in the NEURON environment. It was presented using channelrhodopsin-2 and halorhodopsin to model optical activation and optical silencing in neurons.