Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
4 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-4 of 4
carefully matching biophysical properties of actuators and indicators can permit unambiguous excitation with a single wavelength in a so-called single-beam all-optical experiment
Recent studies ... have used ... GCaMP calcium imaging to interrogate the neural circuitry controlling hormone secretion... in addition to GCaMP imaging of individual cells in vitro and neural populations in vivo using fiber photometry.
Optogenetic actuators, calcium or voltage imaging probes and other molecular tools, combined with advanced microscopies have allowed an "all-optical" readout and modulation of neural circuits.
Closed-loop all-optical neuromodulation is a multi-component optogenetic strategy that combines optical actuators with optical indicators. It is proposed to enable feedback-controlled neuromodulation using light for both perturbation and activity readout.