Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
12 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-12 of 12
we created a modular light-controlled skeletal muscle-powered bioactuator that can generate up to 300 µN (0.56 kPa) of active tension force in response to a noninvasive optical stimulus
Optogenetic control of contractility is a light-based engineering method proposed to spatially modulate cellular contractility and thereby influence cell migration behavior. In a one-dimensional active gel model, optogenetic activation or inhibition of contractility is predicted to switch cells between sessile and motile states at realistic parameter values.
This optogenetic method regulates endogenous nuclear formin mDia by photoreactive release of formin autoinhibition. In the cited Science study, mDia activation induced rapid and reversible nuclear actin network assembly, followed by MAL nuclear accumulation and SRF activity.
The macroscope was implemented with a random-access scanning head capable of drawing arbitrarily-chosen stimulation patterns with sub-millisecond temporal resolution allowing precise epicardial activation of Channelrhodopsin2 (ChR2).
Here, we present a custom two-photon optical system that couples light-sheet imaging and 3D excitation with acousto-optic deflectors for simultaneous high-speed volumetric recording and optogenetic stimulation. By employing a zebrafish line with pan-neuronal expression of both the calcium reporter GCaMP6s and the red-shifted opsin ReaChR, we implemented a crosstalk-free, noninvasive all-optical approach...
A recently developed technology, termed optogenetic functional magnetic resonance imaging (ofMRI), offers a possible solution. By combining targeted activation of transplanted neurons with large-scale, noninvasive measurements of brain activity, ofMRI can directly visualize the effect of engrafted neurons firing on downstream regions.
An optrode, also called an optode, is a neural probe that integrates conventional electrophysiological recording with one or more optical channels for optogenetic activation in live animals. It is used to deliver light to neural tissue while measuring electrical activity from the same preparation.
ChRmine is only mentioned in the supplied evidence as a comparator in the context of pump-fold channelrhodopsin structural analysis. The provided source does not directly describe ChRmine’s sequence, engineering, or functional performance.
Intersectional cell type-specific and input-output defined optogenetic activation of this ACB-LHA-LHb pathway can progressively induce a negative behavioral state that depends on Esr1+ LHA-LHb neural activity.
OptoProfilin is an optically activated profilin-based, single-component biosensor reported in 2023. It was described as an optically triggered sensor of applied cellular stress in select immortalized cell lines and presented as the first profilin-based optogenetic biosensor for stress-induced cytoskeletal changes.
The supplied web research summary explicitly names optogenetic reactivation as an engram manipulation approach used in a directly relevant retrosplenial cortex study.
we developed an optogenetic tool named photoactivatable CaMKII (paCaMKII) by fusing a light-sensitive domain (LOV2) to CaMKIIα