Toolkit Items

Lattice lightsheet microscopy (LLSM) is a modified light-sheet imaging platform used for three-dimensional optogenetic activation with subcellular resolution. In the cited 2022 study, it enabled high-spatiotemporal-resolution manipulation of cellular behavior, including membrane ruffling and guided cell migration.

The Bessel beam stimulation source integrated into lattice light-sheet microscopy (LLSM) is an optical assay configuration that adds Bessel-beam-based optogenetic stimulation to LLSM without changing the microscope optical configuration. It enables three-dimensional photoactivation with improved spatiotemporal control and has been used to manipulate subcellular membrane ruffling and guide cell migration.

We review recently developed functional mapping methods that use optogenetic single-point stimulation in the rodent brain and employ cellular electrophysiology, evoked motor movements, voltage sensitive dyes (VSDs), calcium indicators, or functional magnetic resonance imaging (fMRI) to assess activity.

The merging of optogenetics and optical mapping techniques for 'all-optical' electrophysiology marks a significant step forward. This combination allows for the contactless actuation and sensing of cardiac electrophysiology, offering unprecedented spatial-temporal resolution and control.

The supplied web research summary describes projection-specific manipulation as a central strategy across the anchor review and linked primary papers for dissection of pathways such as BNST→VTA and VTA→NAc/mPFC.

Here, we review recent findings showing the existence of specific neuronal events during both latent and chronic states, and how optogenetic activation of specific cell populations modulate spontaneous seizures.

An optogenetic variant of the classical kindling model of epileptic seizures, in which activatable cells are genetically defined, is called optokindling.