Toolkit Items

optofYap is an optogenetic YAP construct reported in EMBO Reports (2022) for light-responsive control of YAP function in zebrafish embryos. The supplied evidence indicates that it can modulate target gene expression in vivo, but does not provide construct architecture or photoreceptor details here.

Automated optogenetic feedback control is an engineering method that combines light-driven optogenetic regulation with a feedback loop to control gene expression and cell growth. The available evidence supports it as a control framework for precise and robust regulation rather than as a single molecular reagent.

The Light-Activated Degron is a light-responsive construct pattern used for optogenetic control of gene expression in combination with Cryptochrome 2. The available evidence indicates that it enables regulation of gene expression through light-dependent control of protein stability.

The LOV2 module is a light-inducible protein domain incorporated into the FERM domain of an engineered focal adhesion kinase (FAK). In the reported 2021 Nature Communications system, it provides optogenetic input to an allosterically regulated single-protein two-input OR gate while preserving overall FAK domain architecture.

Nanobody-mediated proteolysis-targeting chimeras are a degradation-based engineering method used in an optogenetically coordinated platform to regulate the intracellular factor Survivin in cancer cells. In the cited study context, they are combined with split-Cas9-based targeted gene editing for multi-level control of cancer cell fate.

Split-Cas9-based targeted gene editing is a gene-editing component used in an optogenetically coordinated platform with nanobody-mediated proteolysis-targeting chimeras. In the reported application, it was used to regulate Survivin as part of a multi-level strategy to control cancer cell fate.

Novel opto-kinases engineered from photoreceptors are light-responsive kinase constructs created by engineering kinase control strategies on the basis of photoreceptor components. They are described as an optogenetic approach for regulating cellular signaling through light-dependent control of protein kinase activity.

We engineered OREC, an orthogonal platform integrating chemogenetic and optogenetic modalities for precise, reversible, multiplex gene control.