Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Closed loop optogenetic compensation (CLOC) is an engineering methodology that monitors pathway output in real time and computes an optogenetically driven transcriptional input to compensate for deletion of a feedback regulator. It was applied to the Saccharomyces cerevisiae pheromone response pathway to define the dynamic requirements of feedback control.
The Cry2-CIB1 light-inducible binding switch is an optogenetic protein interaction module based on cryptochrome 2 and cryptochrome-interacting basic helix-loop-helix 1. In the cited work, it was integrated into a photoactivatable Tet OFF/ON system to regulate expression of a gene of interest using light illumination together with drug application.