Cj4Cas9
Construct PatternAmong these, Cj4Cas9 stood out as particularly noteworthy due to its compact genome size (985 amino acids) and unique PAM preference (5'-NNNGRY-3').
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
Toolkit Items
Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
3 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
▾Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 1
Mechanisms
Layer 2
Architectures
▾Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 2
Architectures
Layer 3
Components
▾Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Layer 3
Components
Technique Branch
Layer 1
Approaches
▾High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 1
Approaches
Layer 2
Methods
▾Concrete methods used to design, build, verify, or characterize engineered systems.
Layer 2
Methods
Sort
pam-dependent target recognition
Showing 1-3 of 3
Loaditems
Page 1 / 1
enCj4Cas9
Construct PatternSeeking to further expand its utility, we engineered Cj4Cas9 for higher activity by introducing L58Y/D900K mutations, resulting in a variant termed enCj4Cas9. This variant exhibits a two-fold increase in nuclease activity compared to the wild-type Cj4Cas9 and recognizes a simplified N3GG PAM, considerably expanding its targeting scope.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
WT-SpCas9
Engineering MethodWT-SpCas9 is the widely used Streptococcus pyogenes Cas9 nuclease used for CRISPR genome editing. In the supplied evidence, it is defined by a requirement for an NGG protospacer adjacent motif for target recognition.
CFBacMamMusHumTxRep
Ev 28Rep 9Pr 71
Page 1 / 1