Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
2 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-2 of 2
Polyamidoamine (PAMAM) carriers spanning generations G0-G7 were characterized as polymeric nucleic acid delivery formulations for light-directed delivery in vitro using photochemical internalization (PCI). In an OHS EGFP osteosarcoma model, PAMAM G3-G7 supported efficient light-directed siRNA silencing, whereas EGFP mRNA up-regulation was not detected after EGFP mRNA/PAMAM transfection with or without PCI.
The paper introduces orthogonal photoactivable upconversion nanoparticle superballs (OP-SBs) assembled from two distinct UCNP modules, enabling 980 nm-triggered ZnPc-mediated singlet-oxygen generation for photochemical internalization/PDT and 808 nm-triggered azobenzene-cap opening for siRNA release.