Toolkit Items

Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.

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Mechanism Branch

Layer 1

Mechanisms

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Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.

Layer 2

Architectures

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Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.

Layer 3

Components

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Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.

Technique Branch

Layer 1

Approaches

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High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.

Layer 2

Methods

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Concrete methods used to design, build, verify, or characterize engineered systems.

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photochemical uncaging or photoactivation of an erythromycin derivative

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MphR(A)/PmphR photochemical gene switch

Multi-Component Switch
ligand-dependent derepression of the pmphr promoter by mphr(a)photochemical uncaging or photoactivation of an erythromycin derivativetranscriptional regulation

The MphR(A)/PmphR photochemical gene switch is an engineered bacterial transcription system in which the MphR(A)/PmphR regulatory module is coupled to a light-activatable erythromycin derivative. In E. coli, photoactivation of the erythromycin derivative enables ligand-dependent activation of transcription from the PmphR-controlled output.

CFBacMamMusHumTxRep
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