Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
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Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
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Arabidopsis phototropins phot1 and phot2 are blue-light receptor proteins from Arabidopsis thaliana that optimize photosynthetic efficiency. Targeted mutagenesis of these photoreceptors can tune photocycle lifetime and thereby alter the duration of receptor activation in planta.
Targeted mutagenesis of Arabidopsis phototropins is an engineering method that tunes phototropin photocycle lifetime to shorten or prolong light-activated receptor signaling. In vitro, mutagenesis altered photocycle lifetime, and in planta slow-photocycling phot1 or phot2 variants extended activation and increased biomass under low-light conditions.